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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Percoll Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Discontinuous Percoll Gradient, supplied by Matos labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Goscripttm Reverse Transcription Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
80% (V/V) Percoll, supplied by AMRAD Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Pharmacia Biotech Ltd percoll solution
Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Percoll Solution, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Percoll, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science percol 25% v/v
Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
Percol 25% V/V, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc v v percoll amersham pharmacia biotech
Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
V V Percoll Amersham Pharmacia Biotech, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck & Co v v 4 percoll
Figure 4. In vivo development <t>of</t> <t>cryopreserved</t> one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and <t>PEPeS</t> for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.
V V 4 Percoll, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. In vivo development of cryopreserved one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and PEPeS for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.

Journal: Scientific reports

Article Title: Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes.

doi: 10.1038/s41598-023-47394-0

Figure Lengend Snippet: Figure 4. In vivo development of cryopreserved one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and PEPeS for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.

Article Snippet: Composition of cryopreservation solution and warming solution Embryos were cryopreserved using PEPeS (10% v/v propylene glycol, 30% v/v ethylene glycol, 0.3 M sucrose, and 20% v/v Percoll [GE Healthcare, Uppsala, Sweden] in phosphate-buffered saline 1 [PB1]) developed by Eto et al.24 for the cryopreservation of rat 2-cell embryos34,35.

Techniques: In Vivo, Derivative Assay